CRL-1573 HEK-293 人胚肾细胞系
简单介绍
CRL-1573 HEK-293 人胚肾细胞系的详细介绍
CRL-1573 HEK-293 人胚肾细胞系
Designations:
HEK-293
Depositors: FL Graham
Biosafety Level:2 [Cells co
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Homo sapiens
Morphology:epithelial
Source:Organ: embryo
Permits/Forms:In addition to the MTA mentio
Restrictions:These cells are distributed for research purposes only. 293 cells, their products, or their derivatives may not be distributed to third parties.
Applications:efficacy testing
transfection host
viruscide testing
Receptors:vitronectin, expressed
Tumorigenic:YES
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19CRL-1573 HEK-293 人胚肾细胞系
Cytogenetic Analysis:
This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.
Age: fetus
Comments:
Although an earlier report suggested that the cells co
The line is excellent for titrating human adenoviruses.
The cells express an unusual cell surface receptor for vitro
The Ad5 insert was clo
Propagation:
ATCC complete growth medium: The ba
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Duration: The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37C.
Subculturing:
Protocol: 1. Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that co
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.An inoculum of 2 x 103 to 6 x 103 viable cells/cm2 is recommended.
6. Incubate cultures at 37°C.6. Subculture when cell co
CRL-1573 HEK-293 人胚肾细胞系
Subcultivation Ratio: 1:6 to 1:10 weekly
Medium Renewal: Every 2 to 3 days
Preservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:
Recommended medium (without the additio
Derivative: ATCC CRL-10852
Derivative: ATCC CRL-12006
Derivative: ATCC CRL-12007
Derivative: ATCC CRL-12013
Derivative: ATCC CRL-12479
Derivative: ATCC CRL-2029
Derivative: ATCC CRL-2368
Purified DNA: ATCC CRL-1573D
References: CRL-1573 HEK-293 人胚肾细胞系
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21631: Da Costa LT, et al. Co
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22319: Graham FL, et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220
22699: Harrison T, et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862
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27819: Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton o
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32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha geneex
32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
32490: Stauderman KA, et al. Characterization of human recombinant neuro
32514: Bartz SR, et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659 CRL-1573 HEK-293 人胚肾细胞系
32726: Sandri-Goldin RM, Hibbard MK. The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-sm antiserum, and the C terminus appears to be required for this interaction. J. Virol. 70: 108-118, 1996. PubMed: 8523514
32829: Ansieau S, et al. Tumor necrosis factor receptor-associated factor (TRAF)-1, TRAF-2, and TRAF-3 interact in vivo with the CD30 cytoplasmic domain; TRAF-2 mediates CD30-induced nuclear factor kappa B activation. Proc. Natl. Acad. Sci. USA 93: 14053-14058, 1996. PubMed: 8943059
32893: Zhang J, et al. Dynamin and beta-arrestin reveal distinct mechanisms for G protein-coupled receptor internalization. J. Biol. Chem. 271: 18302-18305, 1996. PubMed: 8702465
32914: Oppermann M, et al. Mo
32921: Xia Y, et al. Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proc. Natl. Acad. Sci. USA 93: 6770-6774, 1996. PubMed: 8692893
32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224
32971: Uebele VN, et al. Functio
33003: Abell A, et al. Deletions of portions of the extracellular loops of the lutropin/choriogo
33010: Tiberi M, et al. Differential regulation of dopamine D1A receptor respo
33022: Shahrestanifar M, et al. Studies on inhibition of mu and delta opioid receptor binding by dithiothreitol and N-ethylmaleimide. His223 is critical for mu opioid receptor binding and inactivation by N-ethylmaleimide. J. Biol. Chem. 271: 5505-5512, 1996. PubMed: 8621408
33035: Boring L, et al. Molecular clo
33036: Noo
33050: Fox JC, Shanley JR. Antisense inhibition of basic fibroblast growth factor induces apoptosis in vascular smooth muscle cells. J. Biol. Chem. 271: 12578-12584, 1996. PubMed: 8647868
33056: Lee MJ, et al. The inducible G protein-coupled receptor edg-1 signals via the Gi/mitogen-activated protein kinase pathway. J. Biol. Chem. 271: 11272-11279, 1996. PubMed: 8626678
33123: Marchand P, et al. Cysteine mutations in the MAM domain result in mo
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