CRL-2285 Super Tube 狗肾细胞 ATCC

CRL-2285 Super Tube 狗肾细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和ZUI优培养条件！

CRL-2285 Super Tube 狗肾细胞

ATCC® Number:	CRL-2285™
Designations: Super Tube Depositors: RJ Klebe Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Canis familiaris Morphology: epithelial Source: Organ: kidney Disease: normal Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Age: adult Gender: female Comments: Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate). For tubule formation, the cells should be fed twice a day by a complete medium change. Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK. Propagation: ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10 fetal bovine serum. Temperature: 37.0°C Subculturing: Protocol: Remove medium, and rinse with 0.25 trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium 95; DMSO, 5 Storage temperature: liquid nitrogen vapor temperature Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-34 derived from same cell line:ATCC CRL-2286 References: 38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715

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人结肠直肠腺癌 COLO 320HSR CCL-220.1
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CRL-2285 Super Tube 狗肾细胞
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人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854
人胰腺导管腺癌细胞 PL45 CRL-2558
人胰腺癌细胞 Panc 05.04 CRL-2557
人胰腺癌细胞 Panc 04.03 CRL-2555
人胰腺癌细胞 Panc 02.03 CRL-2553
人胰腺癌细胞 Panc 08.13 CRL-2551
人胰腺癌细胞 Panc 03.27 CRL-2549
人胰腺癌细胞 Panc 10.05 CRL-2547
人肝癌细胞 SNU-398 CRL-2233
人成神经瘤细胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌细胞 HPAC CRL-2119
人胰腺癌细胞 HPAF-II CRL-1997
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人脐静脉内皮细胞 HUV-EC-C CRL-1730
人黑素瘤细胞 WM-266-4 CRL-1676
人膀胱癌细胞 HT-1376 CRL-1472
人正常前列腺上皮细胞 RWPE-2 CRL-11610
人正常前列腺上皮细胞 RWPE-1 CRL-11609
鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203
人乳腺癌细胞 MDA-MB-231 HTB-26
小鼠巨噬细胞 RAW 264.7 TIB-71
人横纹肌肉瘤细胞 A-204 HTB-82
人胰腺癌细胞 Capan-2 HTB-80
人胰腺癌细胞 Capan-1 HTB-79
人乳腺导管癌细胞 MDA-MB-435S HTB-129
人胃癌细胞 Hs 746T HTB-135
人肺腺癌细胞 NCI-H441 [H441] HTB-174
人骨髓瘤细胞 U266 TIB-196
人结肠癌细胞 NCI-H716 [H716] CCL-251
人多形性恶性胶质瘤 T98G [T98-G] CRL-1690
人神经母细胞瘤细胞 BE(2)-C CRL-2268
人肾透明细胞癌细胞 Caki-2 HTB-47
人皮肤成纤维细胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤细胞系
CRL-5826 NCI-H226 人肺鳞癌细胞系
Tca-8113 人舌鳞癌细胞
MA-891 小鼠乳腺癌高转移细胞
U14 小鼠宫颈癌细胞
CRL-9518 L5178Y 小鼠淋巴瘤细胞
HTB-112 HEC-1-A 人子宫内膜腺癌细胞
HTB-113 HEC-1-B 人子宫内膜腺癌细胞
AN3 CA 人子宫内膜腺癌细胞
ROS1728 大鼠成骨细胞
CRL-6323 B16F1 小鼠黑色素瘤细胞
HTB-58 SK-MES-1 人肺鳞癌细胞
CCL-127 IMR-32 人神经母细胞瘤细胞
HTB-79 Capan-2 人胰腺癌细胞
BALL-1 人B淋巴细胞急性白血病细胞
CRL-2922 Eayh926 人脐静脉细胞融合细胞
WB-F344 大鼠肝干细胞
CRL-1715 TM4 正常小鼠睾丸细胞
CRL-2539 4T1 小鼠乳腺癌细胞
CRL-1442 BRL-3A 大鼠肝细胞
BRL 大鼠肝细胞
RSC96大鼠雪旺细胞
Isk 人子宫内膜癌细胞
HTB-113 hec-1-b 人子宫内膜腺癌细胞
PLA-802?人恶性横纹肌肉瘤
Pt-K2 袋鼠肾细胞
BS-C-1 非洲绿猴肾细胞
A498人肾癌细胞
3AO人卵巢癌细胞
iec-6 大鼠小肠隐窝上皮细胞
u87MG 人脑星形胶质母细胞瘤
Renca 小鼠肾癌细胞
nci-h929 骨髓瘤细胞
P388小鼠白血病细胞
opm-2 骨髓瘤细胞
MDA-MB-435 人乳腺癌高转移细胞
KU812 人外周血嗜碱性白细胞
NIH:OVCAR-3 人卵巢癌细胞
SW 1990人胰腺癌细胞系
S180人结肠腺癌细胞
HuTu-80十二指肠腺癌
RL95-2 人子宫内腺癌细胞
NCI-H446 人小细胞肺癌细胞
MSTO-211H 人肺癌细胞
H4-Ⅱe 大鼠肝癌细胞系
HLF 人肺成纤维样细胞
LS 180人结肠腺癌细胞
HuTu-80十一指肠腺癌
ST猪睾丸细胞系
REH人急性非B非T淋巴细胞白血病
NCI-H520 人肺鳞癌细胞
RKO 人结肠腺癌细胞
293A 人胚肾细胞
RK-13 兔肾细胞系