CRL-2110 MLE 12 小鼠肺上皮细胞
简单介绍
CRL-2110 MLE 12 小鼠肺上皮细胞的详细介绍
MLE 12 (ATCC® CRL-2110™)
Organism Mus musculus, transgenic, mouse, transgenic
Tissue lung
Cell Type epithelial; SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells co
Age 5 mo
Gender female
Strain FVB/N
Storage Conditions liquid nitrogen vapor phase MLE 12 小鼠肺上皮细胞
Derivation
This line was established in 1992 by Kathryn A. Wikenheiser from pulmo
Clinical Data
female
5 months
Genes Expressed
lung surfactant proteins B and C (SP-B, SP-C)
Tumorigenic Yes
Effects
Yes, tumors were generated within 6 to 9 mo
Comments
The cells express the mRNA for large T antigen.
Lung surfactant proteins B and C were detected.
The cells secrete phospholipids in respo
MLE 12 小鼠肺上皮细胞
HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:
Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
Insulin 0.005 mg/ml
Transferrin 0.01 mg/ml
Sodium selenite 30 nM
Hydrocortisone 10 nM
beta-estradiol 10 nM
HEPES 10 mM
L-glutamine 2 mM (in addition to that in the ba
fetal bovine serum 2%
Subculturing MLE 12 小鼠肺上皮细胞
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation MLE 12 小鼠肺上皮细胞
Freeze medium: Complete growth medium supplemented with 5% (v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Co
Atmosphere: air, 95%; carbon dioxide (CO2), 5% MLE 12 小鼠肺上皮细胞CRL-2110 MLE 12 小鼠肺上皮细胞CRL-2110 MLE 12 小鼠肺上皮细胞CRL-2110 MLE 12 小鼠肺上皮细胞
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